Alomone/Anti-α2A-Adrenergic Receptor (extracellular) Antibody/AAR-020/50 µl,Alomone,,Alomone,Alomone Labs,Alomone G蛋白偶联受体GPCR Alomone激动剂,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Alomone/Anti-α2A-Adrenergic Receptor (extracellular) Antibody/AAR-020/50 µl

产品编号：AAR-020-50µl

市场价：¥7340.00

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>受体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$367.00

品牌： Alomone

公司：Alomone Labs

公司分类：

商品介绍

Cat#:AAR-020

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:m,r

Mayalsoworkin:h

Immunogen

Peptide(C)DAGNSSWNGTEAPG,correspondingtoaminoacidresidues7-20ofratα_2A-adrenoceptor(AccessionP22909).Extracellular,N-terminus.

Anti-α2A-Adrenergic Receptor (extracellular) Antibody -

Accession(Uniprot)NumberP22909

GeneID25083

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyMouse-identical;human-13/14aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.85mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1μgpeptideper1μgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ih,wb

Mayalsoworkin:ic,ifc,ip,lci

Westernblot

Westernblotanalysisofmouse(lanes1and3)andrat(lanes2and4)brainlysates:

1,2.Anti-α_2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:200).
3,4.Anti-α_2A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

Expressionofα_2A-Adrenoreceptorinratcerebellum

ImmunohistochemicalstainingofratcerebellumfreefloatingfrozensectionusingAnti-α_2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020).A.α_2A-Adrenoreceptorstaining(green)appearsinprocessesofBergmannglia(whitearrows)inthemolecularlayer(ML).B.Stainingofthesamesectionwithmouseantiglialfibrillaryacidicprotein(GFAP)(red),demonstratesexpressioninbothBergmanngliaandingranulelayer(GL)astrocytes.C.MergedimageofpanelAandBdemonstratingthatα_2A-adrenoreceptorexpressionisrestrictedtoBergmannglia.DAPIisusedasthecounterstain(blue).

Expressionofα_2A-Adrenoreceptorinrathypothalamus

ImmunohistochemicalstainingofratventromedialhypothalamusfreefloatingfrozensectionusingAnti-α_2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:250).A.α_2A-Adrenoreceptorstaining(green)appearsinastrocyticfibers(arrows)originatingfromthewallofthe3rdventricle(3rdV).B.DAPIisusedasthecounterstain(blue).C.MergedimageofpanelsAandB.

References

IUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
CivantosCalzada,B.andAleixandredeArtinano,A.(2001)Pharmacol.Res.44,195.
Bylund,D.B.(2005)Br.J.Pharmacol.144,159.

Scientificbackground

Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.

AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularN-terminus,andacytoplasmicC-terminus.

Adrenoceptorsaredividedintothreetypes:α₁,α₂ andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α_1A,α_1B,α_1D,α_2A,α_2B,α_2C,β₁,β₂,β₃^1,2.Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem^1,2.

Ingeneral,thepharmacologicalpropertiesofeachsubtypearequitehomogenousacrossdifferentspecies.However,thisisnotthecasefortheα_2A subtypewhichwasfirstisolatedfromhumanplatelets³.Thissubtypeshowsdifferentpharmacologicalpropertiesfromthatofmouseandrat.Forthisreason,untilmoleculartechniquessignificantlyadvanced,itwasbelievedthathumanα_2A andthatofmouseandrat(termedα_2D)weretwodifferentsubtypes.Today,itisacceptedthatthesetwosubtypesareinfactonegeneproductandisgenerallytermedα_2A.Oneofthemaindifferencesbetweenthissubtypefromthedifferentorganismsisitsaffinityforyohimbineandrauwolscine;ratandmouseα_2A displayslessaffinitycomparedtohuman^3,4.

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:04/11/2018

Anti-α_2A-AdrenergicReceptor(extracellular)Antibody(#AAR-020)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα_2A-adrenoceptorfrommouse,rat,andhumansamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

Westernblotanalysisofmouse(lanes1and3)andrat(lanes2and4)brainlysates:

1,2.Anti-α_2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:200).
3,4.Anti-α_2A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone成立于1989年，为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂)，G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便.

公司介绍

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产品分类

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Adenosine Receptors Adhesion Class GPCRs Acid-sensing G-protein Coupled Receptors Adrenergic Receptors

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