Anti-β1-AdrenergicReceptor(extracellular)Antibody(#AAR-023)isahighlyspecificantibodydirectedagainstanextracellularepitopetheratβ1-adrenoceptor.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.Theantibodyrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.Ithasbeendesignedtorecognizeβ1ARfrommouse,ratandhumansamples.
产品编号：AAR-023-0.2ml
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商品介绍
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:m,r
Mayalsoworkin:h
Immunogen
Peptide(C)RAESDEARR(S)YNDP,correspondingtoaminoacidresidues200-214ofratADRB1 (Accession P18090).2nd extracellularloop.
Accession(Uniprot)NumberP18090
GeneID64508
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat,Mouse,human-14/15aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.85mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1µgpeptideper1µgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ih,wb
Mayalsoworkin:ic,ifc,ip,lci
Westernblot
Westernblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5)andratlung(lanes3and6)membranes:
1-3. Anti-β1-AdrenergicReceptor(extracellular) Antibody(#AAR-023),(1:200).
4-6.Anti-β1-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
4-6.Anti-β1-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
Immunohistochemistry
Expressionofβ1-Adrenoceptorinratandmousebrain
Immunohistochemicalstainingofratdorsalraphenucleusandmousecerebellumusing Anti-β1-AdrenergicReceptor(extracellular) Antibody(#AAR-023),(1:400). A. β1-Adrenoceptorstaining(red) inratdorsalraphenucleusappearsinneurons(horizontalarrows). C. β1-Adrenoceptor inmousecerebellumstaining(red)isdetectedingranulecells(horizontalarrows)andinthePurkinjecelllayer(verticalarrow). B,D.NuclearstainingusingDAPIasthecounterstain(blue).
References
- Gilbert,C.E.etal.(2014)Int.J.Mol.Sci.15,2929.
- Woo,A.Y.etal.(2012)ActaPharmacol.Sin.33,335.
- Vicco,M.H.etal.(2014)Can.J.Cardio.30,332.
- Wallukat,G.etal.(2002)Herz27,683.
- Kamal,F.A.etal.(2014)Circ.Res.114,742.
Scientificbackground
β-Adrenergicreceptors(βAR)belongtothesuperfamilyof G-proteincoupledreceptors (GPCRs).βARstimulationbythesympatheticnervoussystemorcirculatingcatecholaminesisinvolvedinperipheralbloodcirculation,metabolicregulation,musclecontraction,andcentralneuralactivities.Intheheart,acuteβARstimulationservesasthemostpowerfulmeanstoregulatecardiacoutputinresponsetoafight-or-flightsituation.
TherearethreeβARsubtypes,β1AR, β2AR and β3AR,incardiacmyocytes1.Thereceptorsconsistofsevenmembrane-spanningdomains,threeintra-andthreeextracellularloops,anextracellularN-terminaldomain,andanintracellularC-terminaltail2.
β1ARisthepredominantreceptorsubtypeexpressedinhumancardiomyocytes.β1ARisalsoexpressedinthekidney3.IthasbeenimplicatedinADIpogenesisandmemoryformation4.
β1ARhasbeenwidelyconnectedwithheartdisease.Boththedownregulationandoverstimulationofβ1ARhavebeenconnectedtomyocyteapoptosis.Inaddition,chronicheartfailureischaracterizedbyabnormalitiesinβ1ARsignaltransduction.ItwasdemonstratedthatheartfailureisalsoinducIBLeinanimalmodelsbyoverexpressionofcomponentsoftheβ1ARsignaltransductioncascade.Transgenicmiceoverexpressingβ1ARdevelop,withaging,heartfailuresimilartothatobservedindilatedcardiomyopathyinhumans5.
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:26/12/2018
Forresearchpurposesonly,notforhumanuse
Westernblot
Westernblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5)andratlung(lanes3and6)membranes:
1-3. Anti-β1-AdrenergicReceptor(extracellular) Antibody(#AAR-023),(1:200).
4-6.Anti-β1-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
4-6.Anti-β1-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
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