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产品名称：Alomone/Anti-Adenosine A3 Receptor Antibody/AAR-004/25 µl

产品编号：AAR-004-25µl

市场价：¥4940.00

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>受体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$247.00

品牌： Alomone

公司：Alomone Labs

公司分类：

商品介绍

Cat#:AAR-004

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,r

Mayalsoworkin:m

Immunogen

Peptide(C)KETGAFYGREFKTAK,correspondingtoaminoacidresidues216-230ofhumanA₃AR(AccessionP0DMS8).3^rdintracellularloop.

Accession(Uniprot)NumberP0DMS8

GeneID140

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyRat,mouse -14/16aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25μl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.7mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1μgpeptideper1μgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ic,ih,wb

Mayalsoworkin:ifc,ip

Westernblot

WesternblotanalysisofMalme-3Mcelllysate:

1.Anti-Adenosine A₃ ReceptorAntibody(#AAR-004),(1:200).
2.Anti-AdenosineA₃ ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

Anti-Adenosine A3 Receptor Antibody - Expression of Adenosine A3 Receptor in rat lung

ExpressionofAdenosine A₃ Receptorinratlung

Immunohistochemicalstainingofparaffin-embedded ratlungsectionsusingAnti-Adenosine A₃Receptor Antibody(#AAR-004),(1:50).Stainingisspecificforrespiratoryepithelialcellsofthebronchiole(arrows)andpneumonocytesinthealveolarwall(a).Hematoxilinisusedasthecounterstain.

Immunocytochemistry

Anti-Adenosine A3 Receptor Antibody - Expression of Adenosine A3 Receptor in a human melanoma cell line

ExpressionofAdenosine A₃ Receptorinahumanmelanomacellline

Immunocytochemicalstainingofparaformaldehyde-fixedandpermeABIlized humanmelanomacells(A2058).A,D.CellswerestainedusingAnti-Adenosine A₃Receptor Antibody(#AAR-004),(1:100),followedbygoatanti-rabbitAlexaFluor-555secondaryantibody.B,E.Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342.C.MergedimageofpanelsAandB.F.MergedimageofpanelsDandE.

References

Okusa,M.D.(2002)Am.J.Physiol.RenalPhysiol.282,F10.
Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
Nakata,H.(1989)J.Biol.Chem.264,16545
Baraldi,P.G.etal.(2006)Curr.Med.Chem.13,3467.
Headrick,J.P.etal.(2003)Am.J.Physiol.HeartCirc.Physiol.285,H1797.
MADI,L.etal.(2004)Clin.CancerRes.10,4472.
Ochaion,A.etal.(2007)Ann.Rheum.Dis.66,446.

Scientificbackground

Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.^1,2 Adenosineexertsitsactionviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA₁ Receptor (A₁AR), AdenosineA_2A Receptor (A_2AAR),AdenosineA_2B Receptor (A_2BAR),and AdenosineA₃ Receptor (A₃AR).Thevariousadenosinereceptorscanbedistinguishedonthebasisoftheirdistinctmolecularstructures,distincttissuedistributions,anddifferentialselectivityforadenosineanalogs.^1-4 AllareintegralmembraneproteinsandaremembersoftheGprotein-coupledreceptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,acytoplasmiccarboxylterminus,andathirdintracellularloopimportantinbindingGproteins.^1-3

ExpressionofA₃ARhasbeenreportedinthebrain,kidney,liver,andheart.Itplaysaroleinmodulationofcerebralischemia,asthma,andcellgrowth.Inaddition,recentstudieshaveestablishedacardioprotectiveroleforA₃AR.⁵ ExpressionofA₃ARwasreportedtobeelevatedincanceroustissuesaswellasinauto-immuneinflammatorydiseases.^6,7 ApatenthasbeenfiledfortheuseofA₃ARandA₃ARagoNISTasadiagnosticMarkerfortherapeutictreatmentsofcancerandotherdiseases.

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:26/02/2019

AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanintracellularepitopeofthehumanadenosineA₃ receptor(A₃AR).Anti-Adenosine A₃ReceptorAntibody(#AAR-004)canbeusedinwesternblotanalysis,aswellasimmunocytochemicalandimmunohistocemicalapplications,andhasbeendesignedtorecognizeA₃ARfromhuman,rat,andmousesamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

WesternblotanalysisofMalme-3Mcelllysate:

1.Anti-Adenosine A₃ ReceptorAntibody(#AAR-004),(1:200).
2.Anti-AdenosineA₃ ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone成立于1989年，为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂)，G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便.

公司介绍

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产品分类

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Adenosine Receptors Adhesion Class GPCRs Acid-sensing G-protein Coupled Receptors Adrenergic Receptors

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