Alomone/Anti-α1A-Adrenergic Receptor (extracellular) Antibody/AAR-015/50 µl,Alomone,,Alomone,Alomone Labs,Alomone G蛋白偶联受体GPCR Alomone神经信号传导,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Alomone/Anti-α1A-Adrenergic Receptor (extracellular) Antibody/AAR-015/50 µl

产品编号：AAR-015-50µl

市场价：¥7340.00

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>受体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$367.00

品牌： Alomone

公司：Alomone Labs

公司分类：

商品介绍

Cat#:AAR-015

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,r

Mayalsoworkin:m

Immunogen

PeptideEDETI*SQINEEPG(C),correspondingtoaminoacidresidues171-183ofhumanα_1A-adrenoceptorwithreplacementofcysteine176(C₁₇₆)withserine(*S) (AccessionP35348).2^ndextracellularloop.

Anti-α1A-Adrenergic Receptor (extracellular) Antibody -

Accession(Uniprot)NumberP35348

GeneID148

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyHuman,rat,mouse,bovine-12/13aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.70mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl2µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ih,wb

Mayalsoworkin:ifc,ip,lci

Westernblot

WesternblotanalysisofPC3celllinelysate(lanes1and3)andratbrainmembrane(lanes2and4):

1,2.Anti-α_1A-AdrenergicReceptor(extracellular)Antibody(#AAR-015),(1:200).
3,4.Anti-α_1A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

Expressionofα_1A-Adrenergicreceptorinratcerebellum

ImmunohistochemicalstainingofratcerebellumusingAnti-α_1A-AdrenergicReceptor(extracellular)Antibody(#AAR-015),(1:100).A.α_1A-adrenoceptor(green)appearsinfibersofBergmannglia(trianglepointsatanexample).B.S100β(red),aMarkerofBergmannglia,isstainedinthesamesection.C.Mergeoftheimagesdemonstratesexpressionofα_1A-adrenoceptorinfibersofBergmannglia.α_1A-adrenoceptorisalsoexpressedincellsinthemolecularlayerthatares100β negative(arrowpointsatanexample).DAPIisusedasthecounterstain(blue).

References

IUPHARRECEPTORDATABASE|ADRENOCEPTORS.
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Perez,D.M.etal.(1993)MolPharmacol44,784.
Minneman,K.P.(1988)Pharmacol.Rev.40,87.

Scientificbackground

Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.

AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.

Adrenoceptorsaredividedintothreetypes:α₁,α₂ andβadrenoceptors. Eachtypeisfurtherdividedintoatleastthreesubtypes:α_1A,α_1B,α_1D,α_2A,α_2B,α_2C,β₁,β₂,β₃.^1,2Theyareexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.^1,2

Allα₁-adrenoceptors(α₁-ARs)activatephospholipasesCandA2.³ Inadditiontomobilizingintracellularcalcium,theα₁-ARshavealsobeenshowntoactivatecalciuminfluxviavoltage-dependentand-independentcalciumchannels.⁴

Theα_1A-ARshaveatleastnineknownalternativesplicedisoforms.Theyarethemostabundantadrenergicreceptorinthebrain,andareconsideredpostsynapticreceptors.α_1A-ARsarealsoexpressedintheheart,liverandprostate.

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:05/11/2018

AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanα_1A-adrenergicreceptor.Anti-α_1A-AdrenergicReceptor(extracellular)Antibody(#AAR-015)canbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα_1A-adrenoceptorfromhuman,rat,andmousesamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

WesternblotanalysisofPC3celllinelysate(lanes1and3)andratbrainmembrane(lanes2and4):

1,2.Anti-α_1A-AdrenergicReceptor(extracellular)Antibody(#AAR-015),(1:200).
3,4.Anti-α_1A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone成立于1989年，为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂)，G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便.

公司介绍

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产品分类

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Adenosine Receptors Adhesion Class GPCRs Acid-sensing G-protein Coupled Receptors Adrenergic Receptors

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