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抗体、小分子、蛋白质和肽
℡ 4000-520-616
℡ 4000-520-616
Alomone/Anti-Human Adenosine A2A Receptor (extracellular)-FITC Antibody/AAR-007-F/2 x 50 µl
产品编号:AAR-007-F-50µl
市  场 价:¥10540.00
场      地:美国(厂家直采)
产品分类: 生化试剂>生物分子>受体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$527.00
品      牌: Alomone
公      司:Alomone Labs
公司分类:
Alomone/Anti-Human Adenosine A2A Receptor (extracellular)-FITC Antibody/AAR-007-F/2 x 50 µl
商品介绍
Cat#:AAR-007-F
  • LyophilizedPowder
  • AntigenIncl.
  • ShippedatRoomTemp.
  • Type:Polyclonal
    Source:Rabbit
    Reactivity:h
    Immunogen
    PeptideCGQPKEGKNHSQG,correspondingtoaminoacidresidues146-158ofhumanadenosine A2A receptor(AccessionP29274).Extracellular,2ndloop.
    Accession(Uniprot)NumberP29274
    GeneID135
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyHumanonly.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    SpecificityWillnotrecognizeratormousesamples.
    LabelFluoresceinisothiocyanate(FITC).
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefortwoweeks.Forlongerperiods,itshouldbestoredat-20°C.
    Controlantigenreconstitution100µldoubledistilledwater(DDW).
    Controlantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-007),anddirectflowcytometry(labeledantibody).
    Applications:fc
    Mayalsoworkin:ic,lci
    Directflowcytometry
    DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:
    ___ Cells.
    ___Cells+rabbitIgGisotypecontrol-FITC.
    ___ Cells+Anti-HumanAdenosineA2A Receptor(extracellular)-FITCAntibody (#AAR-007-F),(2.5µg).
    DirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:
    ___ Cells.
    ___Cells+rabbitIgGisotypecontrol-FITC.
    ___ Cells+Anti-HumanAdenosineA2A Receptor(extracellular)-FITC Antibody (#AAR-007-F),(2.5µg).
    References
    1. Okusa,M.D.(2002)Am.J.Physiol.282,F10.
    2. Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
    3. Nakata,H.(1989)J.Biol.Chem.264,16545.
    4. Cunha,R.A.(2001)NeuRochem.Int.38,107.
    5. Fredholm,B.B.etal.(2000)Naunyn-SchmiedebergsArch.Pharmacol.362,364.
    6. Canals,M.etal.(2005)J.Neurochem.92,337.
    7. Ohta,A.andSitkovsky,M.(2001)Nature414,916.
    8. Ohta,A.etal.(2006)Proc.Natl.Acad.Sci.103,13132.
    Scientificbackground

    Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart1,2. Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors): AdenosineA1 Receptor (A1AR),AdenosineA2A Receptor(A2AAR), AdenosineA2B Receptor (A2BAR),and AdenosineA3 Receptor (A3AR).Allareintegralmembraneproteinsandaremembersofthe Gprotein-coupled receptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellular-NH2 terminus,cytoplasmic-COOHterminus,andathirdintracellularloopimportantforbindingGproteins.1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs1-3.

    Adenosinereceptorscontrolneurotransmitterreleasethroughthe facilitatory A2AARandtheinhibitoryA1AR.4 A2AARandA1ARarethemajoradenosinereceptorsubtypesexpressedinthecentralnervoussystem(CNS).A2AARismainlyexpressedinthestriatumonGABAergicstriatopallidalneurons,whileA1ARiswidelydistributedthroughouttheCNS5,6.

    A2AARwassuggestedtoplayacriticalroleinattenuationofsystemicinflammatoryresponsesandpreventionofextensivetissuedamage.7 ItwassuggestedthatextracellularadenosinethataccumulatesininflamedanddamagedtissuemayactivatetheA2AARexpressedinimmunecellsleADIngtotermination/inhibitionoftheimmuneresponse.7 ItwasfurthersuggestedthatthissamemechanismmayprotecttumorsfromantitumorTcellsthroughanimmunosuppressivesignalgeneratedbytheactivationofA2AARonTcellsbyextracellularadenosineproducedfromhypoxiccanceroustissues8.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat

    Checkoutmoreofournewproducts.

    Lastupdate:24/03/2019

    Anti-HumanAdenosineA2A Receptor(extracellular)Antibody (#AAR-007)isahighlyspecificantibodydirectedagainstanepitopeofhumanprotein.Theantibodycanbeusedinwesternblotandlivecellflowcytometryapplications.IthasbeendesignedtorecognizeA2aRfromhumansamplesonly.Theantibodywillnotrecognizethereceptorfrommouseorratsamples.

    Anti-HumanAdenosineA2A Receptor(extracellular)-FITC Antibody (#AAR-007-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.

    Forresearchpurposesonly,notforhumanuse
    Directflowcytometry
    DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:
    ___ Cells.
    ___Cells+rabbitIgGisotypecontrol-FITC.
    ___ Cells+Anti-HumanAdenosineA2A Receptor(extracellular)-FITCAntibody (#AAR-007-F),(2.5µg).
    DirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:
    ___ Cells.
    ___Cells+rabbitIgGisotypecontrol-FITC.
    ___ Cells+Anti-HumanAdenosineA2A Receptor(extracellular)-FITC Antibody (#AAR-007-F),(2.5µg).
    References
    1. Okusa,M.D.(2002)Am.J.Physiol.282,F10.
    2. Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
    3. Nakata,H.(1989)J.Biol.Chem.264,16545.
    4. Cunha,R.A.(2001)Neurochem.Int.38,107.
    5. Fredholm,B.B.etal.(2000)Naunyn-SchmiedebergsArch.Pharmacol.362,364.
    6. Canals,M.etal.(2005)J.Neurochem.92,337.
    7. Ohta,A.andSitkovsky,M.(2001)Nature414,916.
    8. Ohta,A.etal.(2006)Proc.Natl.Acad.Sci.103,13132.
    品牌介绍
    Alomone成立于1989年,为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂),G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证,以冻干粉形式提供,可确保产品生物活性和保质期。Alomone可提供多种包装规格,灵活方便.
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