Alomone/Anti-Human Adenosine A2AReceptor (extracellular) Antibody/AAR-007/25 µl,Alomone,,Alomone,Alomone Labs,Alomone经销商/代理商 Alomone蛋白质,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Alomone/Anti-Human Adenosine A2AReceptor (extracellular) Antibody/AAR-007/25 µl

产品编号：AAR-007-25µl

市场价：¥4940.00

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>受体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$247.00

品牌： Alomone

公司：Alomone Labs

公司分类：

商品介绍

Cat#:AAR-007

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h

Immunogen

PeptideCGQPKEGKNHSQG,correspondingtoaminoacidresidues146-158ofhumanadenosine A_2A receptor(AccessionP29274).2^ndextracellularloop.

Anti-Human Adenosine A2AReceptor (extracellular) Antibody -

Accession(Uniprot)NumberP29274

GeneID135

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyHumanonly.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN_3.

SpecificityWillnotrecognizeratormousesamples.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.8mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefortwoweeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ifc,wb

Mayalsoworkin:ic,ih,ip,lci

Westernblot

WesternblotanalysisofhumanHL-60promyelocyticleukemiacelllinelysate(lanes1and3)andhumanJurkatT-cellleukemiacelllinelysate(lanes2and4):

1,2.Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody(#AAR-007),(1:200).
3,4.Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Indirectflowcytometry

IndirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:

^___ Cells.
^___Cells+goat-anti-rabbit-FITC.
^___Cells+ Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody(#AAR-007),(2.5µg)+goat-anti-rabbit-FITC.

IndirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:

^___ Cells.
^___Cells+goat-anti-rabbit-FITC.
^___Cells+ Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody(#AAR-007),(2.5µg)+goat-anti-rabbit-FITC.

References

Okusa,M.D.(2002)Am.J.Physiol.282,F10.
Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
Nakata,H.(1989)J.Biol.Chem.264,16545.
Cunha,R.A.(2001)NeuRochem.Int.38,107.
Fredholm,B.B.etal.(2000)Naunyn-SchmiedebergsArch.Pharmacol.362,364.
Canals,M.etal.(2005)J.Neurochem.92,337.
Ohta,A.andSitkovsky,M.(2001)Nature414,916.
Ohta,A.etal.(2006)Proc.Natl.Acad.Sci.103,13132.

Scientificbackground

Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart^1,2. Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors): AdenosineA₁ Receptor (A₁AR),AdenosineA_2A Receptor(A_2AAR), AdenosineA_2B Receptor (A_2BAR),and AdenosineA₃ Receptor (A₃AR).Allareintegralmembraneproteinsandaremembersofthe Gprotein-coupled receptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellular-NH₂ terminus,cytoplasmic-COOHterminus,andathirdintracellularloopimportant forbindingGproteins.^1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs^1-3.

Adenosinereceptorscontrolneurotransmitterreleasethroughthe facilitatory A_2AARandtheinhibitoryA₁AR.⁴ A_2AARandA₁ARarethemajoradenosinereceptorsubtypesexpressedinthecentralnervoussystem(CNS).A_2AARismainlyexpressedinthestriatumonGABAergicstriatopallidalneurons,whileA₁ARiswidelydistributedthroughouttheCNS^5,6.

A_2AARwassuggestedtoplayacriticalroleinattenuationofsystemicinflammatoryresponsesandpreventionofextensivetissuedamage.⁷ ItwassuggestedthatextracellularadenosinethataccumulatesininflamedanddamagedtissuemayactivatetheA_2AARexpressedinimmunecellsleADIngtotermination/inhibitionoftheimmuneresponse.⁷ ItwasfurthersuggestedthatthissamemechanismmayprotecttumorsfromantitumorTcellsthroughanimmunosuppressivesignalgeneratedbytheactivationofA_2AARonTcellsbyextracellularadenosineproducedfromhypoxiccanceroustissues⁸.

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:24/03/2019

Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody (#AAR-007)isahighlyspecificantibodydirectedagainstanepitopeofhumanprotein.Theantibodycanbeusedinwesternblotandlivecellflowcytometryapplications.IthasbeendesignedtorecognizeA_2ARfromhumansamplesonly.Theantibodywillnotrecognizethereceptorfrommouseorratsamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

WesternblotanalysisofhumanHL-60promyelocyticleukemiacelllinelysate(lanes1and3)andhumanJurkatT-cellleukemiacelllinelysate(lanes2and4):

1,2.Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody(#AAR-007),(1:200).
3,4.Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone成立于1989年，为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂)，G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便.

公司介绍

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产品分类

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Adenosine Receptors Adhesion Class GPCRs Acid-sensing G-protein Coupled Receptors Adrenergic Receptors

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