Anti-Adenosine A2B Receptor(extracellular)Antibody(#AAR-003)isahighlyspecificantibodydirectedagainstanextracellularepitopeofhumanadenosineA2B receptor(A2BAR).Theantibody canbeusedinwesternblot,immunohistochemistry,livecellimagingandindirectflowcytometryapplications.IthasbeendesignedtorecognizeA2BAR fromhuman,ratandmousesamples.
产品编号：AAR-003-25µl
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商品介绍
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:h,m,r
Immunogen
PeptideKDSATNN*STEPWDGTTNESC,correspondingtoaminoacidresidues147-166ofhuman A2BARwithreplacementofcysteine154(C154)withserine(*S)(AccessionP29275).2ndextracellularloop.
Accession(Uniprot)NumberP29275
GeneID136
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat-14/19aminoacidresiduesidentical;mouse-13/19aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.75mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1µgpeptideper1µgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ic,ifc,ih,lci,wb
Mayalsoworkin:ip
Westernblot
WesternblotanalysisofHL-60(lanes1and2)cellline,ratbrain(lanes3and4)andmousebrain(lanes5and6)lysates:
1,3,5.Anti-Adenosine A2B Receptor (extracellular)Antibody(#AAR-003),(1:200).
2,4,6.Anti-AdenosineA2B Receptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
2,4,6.Anti-AdenosineA2B Receptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
Immunohistochemistry
ExpressionofAdenosine A2B Receptorinratlung
ImmunohistochemicalstainingofratlungparaffinembeddedsectionsusingAnti-Adenosine A2B Receptor(extracellular) Antibody(#AAR-003),(1:50).Stainingispresentintherespiratoryepitheliumofthebronchiole(Br)aswellasinthepneumonocytesofthealveolarwall(alveoli,(A).ColorreactionwasobtainedwithSuperPictureHRP-conjugatedpolymer(Zymed)followedbyDAB.Hematoxilinisusedasthecounterstain.
Indirectflowcytometry
Ratenterochromaffin(EC)cells(1:500),(Damen,R. etal. (2013) PLoSONE 8, e62607.).
Livecellimaging/Immunocytochemistry
ExpressionofAdenosine A2B Receptor inratPC12cells
Cellsurfacedetectionof Adenosine A2B ReceptorinintactlivingratpheochromocytomaPC12cells.A.ExtracellularstainingofcellsusingAnti-Adenosine A2B Receptor(extracellular) Antibody(#AAR-003), (1:100)followedbygoatanti-rabbit-AlexaFluor-594secondaryantibody.B.MergeofAandliveviewofthecells.
References
- Okusa,M.D.(2002)Am.J.Physiol.RenalPhysiol.282,F10.
- Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
- Nakata,H.(1989)J.Biol.Chem.264,16545.
- Baraldi,P.G.etal.(2006)Curr.Med.Chem.13,3467.
- Polosa,R.(2002)Eur.Respir.J.20,488.
Scientificbackground
Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.1,2 Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors): AdenosineA1 Receptor (A1AR), AdenosineA2A Receptor (A2AAR),AdenosineA2B Receptor (A2BAR),and AdenosineA3 Receptor (A3AR).AllareintegralmembraneproteinsandaremembersoftheGprotein-coupledreceptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,acytoplasmiccarboxylterminus,andathirdintracellularloopimportantinbindingGproteins.1-3
Thevariousadenosinereceptorscanbedistinguishedonthebasisoftheirdistinctmolecularstructures,distincttissuedistributions,anddifferentialselectivityforadenosineanalogs.1-4
TheadenosineA2receptorsubtypewassuBDividedaccordingtoaffinityforadenosine;A2AARhashighaffinityandA2BARhaslowaffinity.
A2BARisexpressedinmastcells,neutrophils,monocytes,macrophages,dendriticcells,Tcells,bronchialepithelium,intestinalepithelium,smoothmuscle,brain,andothercells.5 Inintestinalepithelia,A2BARwasfoundtobethemajoradenosinereceptorsubtypeexpressedandisthoughttobeinvolvedindiarrhealdiseases.Itisalsobelievedtobeinvolvedinthepathogenesisofchronicairwayinflammatorydiseases.4 Thus,antagoNISTsofA2BARmightbeeffectiveforthetreatmentofinflammatorygastrointestinaltractdisorders4 aswellasasthmaandchronicobstructivepulmonarydisease.5
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:19/03/2019
Forresearchpurposesonly,notforhumanuse
Westernblot
WesternblotanalysisofHL-60(lanes1and2)cellline,ratbrain(lanes3and4)andmousebrain(lanes5and6)lysates:
1,3,5.Anti-Adenosine A2B Receptor (extracellular)Antibody(#AAR-003),(1:200).
2,4,6.Anti-AdenosineA2B Receptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
2,4,6.Anti-AdenosineA2B Receptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
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