AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanextracellularepitopeoftheratα2C-adrenoceptor.Anti-α2C-AdrenergicReceptor(extracellular)Antibody(#AAR-022)canbeusedforwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα2C-adrenoceptorfromrat,mouseandhumansamples.
产品编号：AAR-022-0.2ml
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商品介绍
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:r
Mayalsoworkin:h,m
Immunogen
Peptide(C)RRPDGAAYPQ*SGLNDE,correspondingtoaminoacidresidues192-207ofratα2C-adrenoceptorwithreplacementofcysteine202(C202)withserine(*S)(Accession P22086).2nd extracellularloop.
Accession(Uniprot)NumberP22086
GeneID24175
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat,mouse-15/16aminoacidresiduesidentical;human-14/16aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.8mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1μgpeptideper1μgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ih,wb
Mayalsoworkin:ic,ifc,ip,lci
Westernblot
Westernblotanalysisofratlung(lanes1and4),kidney(lanes2and5)andbrain(lanes3and6)membranes:
1-3. Anti-α2C-AdrenergicReceptor(extracellular)Antibody(#AAR-022),(1:200).
4-6.Anti-α2C-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
4-6.Anti-α2C-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
Immunohistochemistry
Expressionofα2C-Adrenoreceptorinratkidney
Immunohistochemicalstainingofratkidneyparaffinembeddedsectionsusing Anti-α2C-AdrenergicReceptor(extracellular) Antibody(#AAR-022),(1:100).α2C-Adrenoreceptorisexpressedinbothproximal(PT)anddistal(DT)renaltubules andinthecollectingducts(CD).Hematoxilinisusedasthecounterstain.
References
- IUPHARRECEPTORDATABASE|ADRENOCEPTORS.
- Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
- Murphy,T.J.andBylund,D.B.(1988)J.Pharmacol.Exp.Ther.244,571.
- Blaxall,H.S.etal.(1991)J.Pharmacol.Exp.Ther.259,323.
- BjorklindM.etal.(1999)Neuroscience88,1187.
- TanilaH.etal.(1999)Eur.J.Neurosci.11,599.
Scientificbackground
Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.
AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularN-terminus,andacytoplasmicC-terminus.
Adrenoceptorsaredividedintothreetypes:α1,α2 andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β31,2.Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem1,2.
Theα2C-adrenoceptorsubtypewasidentifiedbybindingstudiesintheopossumkidneyandincelllinesfromthisorgan(OKcells)3,4.
Surprisingly,theα2C-adrenoceptorsubtypeseemsnottoplayamajorroleincardiovascularregulationortheotherclassicaleffectsofα2-adrenergicreceptors5.Someresearchershavesuggestedthattheα2C-adrenoceptorsubtypemayplayaroleinmodulatingmotorbehaviourandperhapsinmemoryprocesses6,7.
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea50µlfreetrialsample!
Lastupdate:01/11/2018
Forresearchpurposesonly,notforhumanuse
Westernblot
Westernblotanalysisofratlung(lanes1and4),kidney(lanes2and5)andbrain(lanes3and6)membranes:
1-3. Anti-α2C-AdrenergicReceptor(extracellular)Antibody(#AAR-022),(1:200).
4-6.Anti-α2C-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
4-6.Anti-α2C-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
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