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抗体、小分子、蛋白质和肽
℡ 4000-520-616
℡ 4000-520-616
Alomone/Anti-α1D-Adrenergic Receptor (extracellular) Antibody/AAR-019/0.2 ml
产品编号:AAR-019-0.2ml
市  场 价:¥9500.00
场      地:美国(厂家直采)
产品分类: 生化试剂>生物分子>受体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$475.00
品      牌: Alomone
公      司:Alomone Labs
公司分类:
Alomone/Anti-α1D-Adrenergic Receptor (extracellular) Antibody/AAR-019/0.2 ml
商品介绍
Cat#:AAR-019
  • LyophilizedPowder
  • AntigenIncl.
  • ShippedatRoomTemp.
  • Type:Polyclonal
    Source:Rabbit
    Reactivity:h,m,r
    Immunogen
    Peptide(C)EPVPPDERF*SGITEE,correspondingtoaminoacidresidues231-245ofratα1D-adrenoceptorwithreplacementofcysteine240(C240)withserine(*S)(AccessionP23944).3rdextracellularloop.
    Accession(Uniprot)NumberP23944
    GeneID29413
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyRat,human,mouse-14/15aminoacidresiduesidentical.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormulationLyophilizedpowder.Reconstitutedanitbodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.8mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Controlantigenreconstitution100µldoubledistilledwater(DDW).
    Controlantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1μgpeptideper1μgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,ifc,ih,lci,wb
    Mayalsoworkin:ip
    Westernblot
    Westernblotanalysisofratbrainstem(lanes1and3),mousebrain(lanes2and4),SH-SY5Y(lanes5and7)andJurkat(lanes6and8)lysates:
    1,2,5,6.Anti-α1D-AdrenergicReceptor(extracellular) Antibody(#AAR-019),(1:200).
    3,4,7,8.Anti-α1D-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
    Immunohistochemistry
    Expressionofα1D-Adrenoceptorinratheart
    ImmunohistochemicalstainingofratheartparaffinembeddedsectionsusingAnti-α1D-AdrenergicReceptor(extracellular)Antibody(#AAR-019),(1:100).α1D-Adrenoceptorisexpressedincardiomyocytesofthemyocardiumandinthesmoothmuscleofthebloodvessels(arrows).Hematoxilinisusedasthecounterstain.
    Expressionofα1D-Adrenoceptorinratneocortex
    Immunohistochemicalstainingofα1D-adrenoceptorinratneocortexusingAnti-α1D-AdrenergicReceptor(extracellular)Antibody(#AAR-019). A.Mostintensestainingofα1D-adrenoceptor(green)appearsinapicaldendrites(thinhorizontalarrows)butalsointhesoma(thickhorizontalarrow).Fewcorticalinterneuronsexpressα1D-adrenoceptor.B.Thesamesectionwasalsostainedforparvalbuminandonecell(markedwithaverticalarrow)alsoexpressesα1D-adrenoceptor.
    Indirectflowcytometry
    IndirectflowcytometryanalysisofintactliveJurkatcells:
    ___Unstainedcells.
    ___Cells+Anti-α1D-AdrenergicReceptor(extracellular) Antibody(#AAR-019),(5-10µgantibody/0.5x106cells).
    Thecontrolantigenisnotsuitableforthisapplication.
    Livecellimaging/Immunocytochemistry
    Expressionofα1D-Adrenoceptor inratPC12cells
    Cellsurfacedetectionof α1D-AdrenoceptorinintactlivingratpheochromocytomaPC12cells.A.ExtracellularstainingofcellsusingAnti-α1D-AdrenergicReceptor (extracellular) Antibody(#AAR-019)(1:100) followedbygoatanti-rabbit-AlexaFluor-594secondaryantibody.B.MergeofAandliveviewofthecells.
    References
    1. IUPHARRECEPTORDATABASE|ADRENOCEPTORS
    2. Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
    3. Chen,Z.J.andMinneman,K.P.(2005)ActaPharmacol.Sin.26,1281.
    4. Tanoue,A.etal.(2002)J.Clin.Invest.109,765.
    5. Chen,Q.etal.(2005)J.Urol.174,370.
    Scientificbackground

    Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.
     
    AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.

    Adrenoceptorsaredividedintothreetypes:α12 andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes: α1A, α1B1D, α2A, α2B, α2C1, β2, β31,2.Theadrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem1,2.
     
    α1-Adrenergicreceptorsplayanimportantroleinthephysiologicalresponsetoepinephrineandnorepinephrine,particularlyinthecardiovascularsystem.Allthreeclonedα1 receptors(α1A1B,andα1D)coupletoGq/11.Whiletheircellulardistributionisclear,theirfunctionalroleislesssoduetoalackofspecificagoNISTs/antagonists3.
     
    Asthesereceptorslackspecificagonists/antagonists,knock-out(KO)studiesinmicehaveshedsignificantlightontheircellularrole.Individualα1-adrenoceptorKOmicedonotdisplayphysicalabnormalitiesingeneral.SimilarKOstudiesindicatethatallα1 subtypesseemtobeinvolvedintheregulationofbloodpressure3.Also,studiesindicatethatα1D-adrenoceptorseemstoplayanimportantroleinaorticcontraction4,renalfunction,nociception,basallocomotoractivity3 aswellasbladdercontraction5

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    receivea50µlfreetrialsample!
    Lastupdate:19/03/2019

    AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanextracellularepitopeoftheratα1D-adrenergicreceptor.Anti-α1D-AdrenergicReceptor(extracellular)Antibody(#AAR-019)canbeusedinwesternblot,indirectflowcytometryandimmunohistochemicalapplications.Ithasbeendesignedtorecognizeα1D-adrenoceptorfrommouse,ratandhumansamples.

    Forresearchpurposesonly,notforhumanuse
    Westernblot
    Westernblotanalysisofratbrainstem(lanes1and3),mousebrain(lanes2and4),SH-SY5Y(lanes5and7)andJurkat(lanes6and8)lysates:
    1,2,5,6.Anti-α1D-AdrenergicReceptor(extracellular) Antibody(#AAR-019),(1:200).
    3,4,7,8.Anti-α1D-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
    品牌介绍
    Alomone成立于1989年,为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂),G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证,以冻干粉形式提供,可确保产品生物活性和保质期。Alomone可提供多种包装规格,灵活方便.
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