Alomone/Anti-EMR1 (extracellular)-FITC Antibody/AER-051-F/50 µl,Alomone,,Alomone,Alomone Labs,进口试剂Alomone直采生命科学Alomone Labs价格优惠,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Alomone/Anti-EMR1 (extracellular)-FITC Antibody/AER-051-F/50 µl

产品编号：AER-051-F-50µl

市场价：¥10540.00

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>受体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$527.00

品牌： Alomone

公司：Alomone Labs

公司分类：

商品介绍

Cat#:AER-051-F

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,m,r

Immunogen

PeptideCKQGFLSSNGQNHFK,correspondingtoaminoacidresidues58-72ofhumanEMR1(Accession Q14246).Extracellular,N-terminus.

Anti-EMR1 (extracellular)-FITC Antibody -

Accession(Uniprot)NumberQ14246

GeneID2015

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyMouse,Rat–11/14aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

LabelFluoresceinisothiocyanate(FITC).

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50µldoubledistilledwater(DDW).

Antibodyconcentrationafterreconstitution1mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefortwoweeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AER-051),anddirectflowcytometry(labeledantibody).

Applications:fc

Mayalsoworkin:ic,lci

Directflowcytometry

DirectflowcytometryofliveintactmouseJ774macrophagecells:

^___ Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).

DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:

^___ Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).

References

Legrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.
McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.
Araç,D.etal.(2012)EMBOJ.31,1364.

Scientificbackground

Humanepidermalgrowthfactor(EGF)–likemodulecontainingmucin-likehormonereceptor1(EMR1)isasurfacereceptorwithunknownfunctionthatbelongstotheadhesionfamilyoftheGprotein-coupledreceptors(GPCR).ThisreceptorisencodedbytheADGRE1andlikeallGPCRs,containsseventransmembranedomains,anintracellularC-terminusandanextracellularN-terminus.TheNH2domainhassixEGF-likedomains.

AdhesionGPCRssharealongN-terminalextracellularsequencescomprisingmultipledomains,separatedfromthetransmembranesegmentsbyaserine/threonine-richdomain,afeaturereminiscentofmucin-like,single-span,integralmembraneglycoproteinswithadhesiveproperties^1,2.

EMR1proteinisahomologofF4/80,amurineproteinthatiswidelybeingusedasaMarkerofmurinemacrophagepopulations¹.

HumanEMR1isexclusivelyexpressedonmatureeosinophilsinthebloodandbonemarrow andinnasalpolyps.Thus,EMR1is consideredtobeapotentialtherapeutictargetforthetreatmentofeosinophilicdisorders^1,3.

LyophilizedPowderControlAntigenIncluded

Lastupdate:20/03/2019

Anti-EMR1(extracellular)Antibody (#AER-051)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblotandlivecellimagingapplications.Itrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.IthasbeendesignedtorecognizeEMR1fromhuman,mouse,andratsamples.

Anti-EMR1(extracellular)–FITCAntibody (#AER-051-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.

Forresearchpurposesonly,notforhumanuse

Directflowcytometry

DirectflowcytometryofliveintactmouseJ774macrophagecells:

^___ Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).

DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:

^___ Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).

References

Legrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.
McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.
Araç,D.etal.(2012)EMBOJ.31,1364.

品牌介绍

Alomone成立于1989年，为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂)，G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便.

公司介绍

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产品分类

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Adenosine Receptors Adhesion Class GPCRs Acid-sensing G-protein Coupled Receptors Adrenergic Receptors

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