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抗体、小分子、蛋白质和肽
℡ 4000-520-616
℡ 4000-520-616
Alomone/Anti-Adenosine A3 Receptor Antibody/AAR-004/50 µl
产品编号:AAR-004-50µl
市  场 价:¥7340.00
场      地:美国(厂家直采)
产品分类: 生化试剂>生物分子>受体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$367.00
品      牌: Alomone
公      司:Alomone Labs
公司分类:
Alomone/Anti-Adenosine A3 Receptor Antibody/AAR-004/50 µl
商品介绍
Cat#:AAR-004
  • LyophilizedPowder
  • AntigenIncl.
  • ShippedatRoomTemp.
  • Type:Polyclonal
    Source:Rabbit
    Reactivity:h,r
    Mayalsoworkin:m
    Immunogen
    Peptide(C)KETGAFYGREFKTAK,correspondingtoaminoacidresidues216-230ofhumanA3AR(AccessionP0DMS8).3rdintracellularloop.
    Accession(Uniprot)NumberP0DMS8
    GeneID140
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyRat,mouse -14/16aminoacidresiduesidentical.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25μl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.7mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Controlantigenreconstitution100µldoubledistilledwater(DDW).
    Controlantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1μgpeptideper1μgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,ih,wb
    Mayalsoworkin:ifc,ip
    Westernblot
    WesternblotanalysisofMalme-3Mcelllysate:
    1.Anti-Adenosine A3 ReceptorAntibody(#AAR-004),(1:200).
    2.Anti-AdenosineA3 ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
    Immunohistochemistry
    ExpressionofAdenosine A3 Receptorinratlung
    Immunohistochemicalstainingofparaffin-embedded ratlungsectionsusingAnti-Adenosine AReceptor Antibody(#AAR-004),(1:50).Stainingisspecificforrespiratoryepithelialcellsofthebronchiole(arrows)andpneumonocytesinthealveolarwall(a).Hematoxilinisusedasthecounterstain.
    Immunocytochemistry
    ExpressionofAdenosine A3 Receptorinahumanmelanomacellline
    Immunocytochemicalstainingofparaformaldehyde-fixedandpermeABIlized humanmelanomacells(A2058).A,D.CellswerestainedusingAnti-Adenosine AReceptor Antibody(#AAR-004),(1:100),followedbygoatanti-rabbitAlexaFluor-555secondaryantibody.B,E.Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342.C.MergedimageofpanelsAandB.F.MergedimageofpanelsDandE.
    References
    1. Okusa,M.D.(2002)Am.J.Physiol.RenalPhysiol.282,F10.
    2. Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
    3. Nakata,H.(1989)J.Biol.Chem.264,16545
    4. Baraldi,P.G.etal.(2006)Curr.Med.Chem.13,3467.
    5. Headrick,J.P.etal.(2003)Am.J.Physiol.HeartCirc.Physiol.285,H1797.
    6. MADI,L.etal.(2004)Clin.CancerRes.10,4472.
    7. Ochaion,A.etal.(2007)Ann.Rheum.Dis.66,446.
    Scientificbackground

    Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.1,2 Adenosineexertsitsactionviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA1 Receptor (A1AR), AdenosineA2A Receptor (A2AAR),AdenosineA2B Receptor (A2BAR),and AdenosineA3 Receptor (A3AR).Thevariousadenosinereceptorscanbedistinguishedonthebasisoftheirdistinctmolecularstructures,distincttissuedistributions,anddifferentialselectivityforadenosineanalogs.1-4 AllareintegralmembraneproteinsandaremembersoftheGprotein-coupledreceptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,acytoplasmiccarboxylterminus,andathirdintracellularloopimportantinbindingGproteins.1-3

    ExpressionofA3ARhasbeenreportedinthebrain,kidney,liver,andheart.Itplaysaroleinmodulationofcerebralischemia,asthma,andcellgrowth.Inaddition,recentstudieshaveestablishedacardioprotectiveroleforA3AR.5 ExpressionofA3ARwasreportedtobeelevatedincanceroustissuesaswellasinauto-immuneinflammatorydiseases.6,7 ApatenthasbeenfiledfortheuseofA3ARandA3ARagoNISTasadiagnosticMarkerfortherapeutictreatmentsofcancerandotherdiseases.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    receivea25µlfreetrialsample!
    Lastupdate:26/02/2019

    AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanintracellularepitopeofthehumanadenosineA3 receptor(A3AR).Anti-Adenosine AReceptorAntibody(#AAR-004)canbeusedinwesternblotanalysis,aswellasimmunocytochemicalandimmunohistocemicalapplications,andhasbeendesignedtorecognizeA3ARfromhuman,rat,andmousesamples.

    Forresearchpurposesonly,notforhumanuse
    Westernblot
    WesternblotanalysisofMalme-3Mcelllysate:
    1.Anti-Adenosine A3 ReceptorAntibody(#AAR-004),(1:200).
    2.Anti-AdenosineA3 ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
    品牌介绍
    Alomone成立于1989年,为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂),G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证,以冻干粉形式提供,可确保产品生物活性和保质期。Alomone可提供多种包装规格,灵活方便.
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