Alomone/Anti-Adenosine A1Receptor Antibody/AAR-006/50 µl,Alomone,,Alomone,Alomone Labs,Alomone厂家直采，平行进口 Alomone小分子,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Alomone/Anti-Adenosine A1Receptor Antibody/AAR-006/50 µl

产品编号：AAR-006-50µl

市场价：¥7340.00

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>受体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$367.00

品牌： Alomone

公司：Alomone Labs

公司分类：

商品介绍

Cat#:AAR-006

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,m,r

Immunogen

Peptide(C)KKVSASSGDPQKYYGKE,correspondingtoaminoacidresidues213-229ofhumanA₁AR(Accession P30542).3^rd intracellularloop.

Accession(Uniprot)NumberP30542

GeneID134

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyMouse,rat,bovine,canisandpig-identical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution25μl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.85mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1μgpeptideper1μgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ic,ih,wb

Mayalsoworkin:ifc,ip

Westernblot

Westernblotanalysisofratbrain(lanes1,3)andratkidney(lanes2,4)lysates:

1,2.Anti-Adenosine A₁ReceptorAntibody(#AAR-006),(1:200).
3,4.Anti-AdenosineA₁ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

Anti-Adenosine A1Receptor Antibody - Expression of Adenosine A1 Receptor in rat cortex

ExpressionofAdenosine A₁Receptorinratcortex

ImmunohistochemicalstainingofratcortexfrozensectionusingAnti-Adenosine A₁Receptor Antibody(#AAR-006).A.A₁AR(green)appearsinneurons(triangles)andinastrocytes(arrows).B.Parvalbuminstaining(red)appearsincorticalinterneurons.C.ConfocalmergeofimagesdemonstratestheexistenceofA₁ARinasubsetof corticalinterneuronsandastrocytes.

Anti-Adenosine A1Receptor Antibody - Expression of Adenosine A1 Receptor in rat medial septum

ExpressionofAdenosine A₁Receptorinratmedialseptum

ImmunohistochemicalstainingofratmedialseptumfrozensectionusingAnti-Adenosine A₁Receptor Antibody(#AAR-006).A.A₁AR(green)appearsinneurons(rightpointingtriangles). B.Parvalbuminstaining(red)appearsinmedialseptalneurons.C.ConfocalmergeofdemonstratesexpressionofA₁ARinasubsetofmedial septalneurons.

Immunocytochemistry

Anti-Adenosine A1Receptor Antibody - Expression of Adenosine A1 Receptor in rat DRG primary culture

ExpressionofAdenosine A₁ ReceptorinratDRGprimaryculture

Immunocytochemicalstainingofparaformaldehyde-fixedandpermeABIlizedratdorsalrootganglion(DRG)primaryculture.
A.Stainingof DRGcellswithAnti-Adenosine A₁ReceptorAntibody(#AAR-006)(1:100),followedbygoatanti-rabbitAlexaFluor-555secondaryantibody.
B.Nuclearstainingofcellsusingthecell-permeabledyeHoechst33342.
C.MergedimagesofAandB.
F.MergedimagesofDandE.
Magnification:
A-C:x20
D-F:x100

References

Okusa,M.D.(2002)Am.J.Physiol.282,F10.
Nakata,H.(1989)J.Biol.Chem.264,16545.
Linden,J.(1991)FASEBJ.5,2668.
Park,K.S.etal.(2001)J.Pharmacol.Exp.Therap.299,501.
Rice,A.M.etal.(2000)Endocrinology141,1442.
LaNoue,K.F.andMartin,L.F.(1994)FASEBJ.8,72.
Synowitz,M.etal.(2006)CancerRes.66,8550.
Lelievre,V.etal.(1998)Eur.J.Pharmacol.341,289.
Khoo,H.E.etal.(1996)CancerLett.106,17.

Scientificbackground

Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.^1,2Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA₁ Receptor(A₁AR), AdenosineA_2A Receptor (A_2AAR), AdenosineA_2B Receptor (A_2BAR),and AdenosineA₃ Receptor (A₃AR).AllareintegralmembraneproteinsandaremembersoftheGprotein-coupledreceptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,acytoplasmiccarboxylterminus,andathirdintracellularloopthatisimportantforbindingGproteins.^1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs.^1-3

A₁ARiswidelydistributedandhasbeenwellcharacterized.HighexpressionofA₁ARisfoundinthebrain(mainlyinthecortex,cerebellum,andhippocampus),dorsalhornofthespinalcord,adrenalgland,andatria,andtoalowerextentinseveralothertissuesincludingADIposetissue,thecolon,andkidney.^2,4

A₁ARmodulatestheactivityofseveralionchannels.ActivationofA₁AR(byadenosine,itsmajoragoNIST)inhibitsN-typeCa²⁺channelsviaavoltage-dependent,pertussistoxin(PTX)-sensitivepathwayinneuronsoftheratmajorpelvicganglia(MPG).⁵

SinceA₁ARisthemostprominentadenosinereceptorinadipocytes,ithasbecomeanaturaltargetforresearchonobesity,whichisamajorhealthproblem.^6,7 ApossIBLeroleincellproliferationandcarcinogenesishasalsobeensuggestedforA₁AR.^8-10

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea25µlfreetrialsample!

Lastupdate:17/02/2019

AlomoneLabsispleasedtointroduceanewantibodydirectedagainsthumanadenosineA₁receptor.Anti-Adenosine A₁ ReceptorAntibody(#AAR-006)canbeusedinwesternblotanalysis,immunocytochemicalandimmunohistochemicalapplications.IthasbeendesignedtorecognizeadenosineA1Rfromrat,mouseandhumansamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

Westernblotanalysisofratbrain(lanes1,3)andratkidney(lanes2,4)lysates:

1,2.Anti-Adenosine A₁ReceptorAntibody(#AAR-006),(1:200).
3,4.Anti-AdenosineA₁ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone成立于1989年，为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂)，G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便.

公司介绍

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产品分类

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Adenosine Receptors Adhesion Class GPCRs Acid-sensing G-protein Coupled Receptors Adrenergic Receptors

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