Alomone/Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody/AGR-043-F/5 x 50 µl,Alomone,,Alomone,Alomone Labs,Alomone钙离子通道 Alomone肽,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Alomone/Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody/AGR-043-F/5 x 50 µl

产品编号：AGR-043-F-50µl

市场价：¥10540.00

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>受体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$527.00

品牌： Alomone

公司：Alomone Labs

公司分类：

商品介绍

Cat#:AGR-043-F

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,m,r

Immunogen

Peptide(C)DYTWNKDNWTFSP,correspondingtoaminoacidresidues72-84ofhumanGPR65(AccessionQ8IYL9).Extracellular,1^stloop.

Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody -

Accession(Uniprot)NumberQ8IYL9

GeneID8477

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyMouse–12/13aminoacidresiduesidentical;rat-11/13aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

LabelFluoresceinisothiocyanate(FITC).

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50µldoubledistilledwater(DDW).

Antibodyconcentrationafterreconstitution1mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AGR-043),anddirectflowcytometry(labeledantibody).

Applications:fc

Mayalsoworkin:ic,lci

Directflowcytometry

DirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:

^___Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody (#AGR-043-F),(5µg).

DirectflowcytometryofliveintactmouseM1myeloidleukemiacells:

^___Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody(#AGR-043-F),(5µg).

References

Justus,C.R.etal.(2013)Front.Physiol.4,354.
Justus,C.R.etal.(2017)J.Transl.Med.15,204.

Scientificbackground

GPR65,alsocalledTDAG8(T-celldeathassociatedgene8protein),isapH-sensingG-proteincoupledreceptor(GPCR)activatedbyacidicextracellularpHthroughtheprotonationofseveralhistidineresiduesinthereceptor’ssequence.GPR65isinvolvedincancercellmetastasisandproliferation,immunecellfunction,inflammation,andbloodvesselformation¹.

HumanGPR65genehasbeenmappedtoalocationthatisassociatedwithTcelllymphomaandleukemiaabnormalities.

GPR65isprimarilyexpressedinimmunecellsandleukocyte-richtissuessuchascirculatingperipheralleukocytes,spleen,thymus,andtonsils.Expressionisalsodetected pain relevantlocisuchasthedorsalrootganglianeuronsandparticularlysmalldiameterneuronsresponsIBLefornociception^1,2.

GPR65hastheABIlitytoaffecttumordevelopmentandgrowth.OverexpressionofGPR65inLewislungcarcinomacellsincreasestumorgrowthinmiceandmayfacilitateresistancetoacidosis-mediatedcelldeath invitro throughproteinkinaseA(PKA)andERKrelatedpathways¹.

KnockdownofGPR65inNCI-H460humannon-smallcelllungcancercellsdecreasescellsurvivalinacidiccondition.AnotherstudyshowsthatactivationofGPR65byacidosiscanpromoteevasionofcellapoptosisunderglutaminestarvationanditsoverexpressionhasbeenreportedtotransformimmortalizedmammaryepithelialcells¹.

LyophilizedPowderControlAntigenIncluded

Lastupdate:20/03/2019

Anti-GPR65(TDAG8)(extracellular)Antibody(#AGR-043)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot, immunohistochemistry,andlivecellflowcytometry.IthasbeendesignedtorecognizeGPR65fromrat,mouse,andhumansamples.

Anti-GPR65(TDAG8)(extracellular)-FITCAntibody(#AGR-043-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.

Forresearchpurposesonly,notforhumanuse

Directflowcytometry

DirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:

^___Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody (#AGR-043-F),(5µg).

DirectflowcytometryofliveintactmouseM1myeloidleukemiacells:

^___Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody(#AGR-043-F),(5µg).

References

Justus,C.R.etal.(2013)Front.Physiol.4,354.
Justus,C.R.etal.(2017)J.Transl.Med.15,204.

品牌介绍

Alomone成立于1989年，为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂)，G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便.

公司介绍

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产品分类

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Adenosine Receptors Adhesion Class GPCRs Acid-sensing G-protein Coupled Receptors Adrenergic Receptors

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Alomone/Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody/AGR-043-F/5 x 50 µl

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