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抗体、小分子、蛋白质和肽
℡ 4000-520-616
℡ 4000-520-616
Alomone/Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody/AAR-003-F/2 x 50 µl
产品编号:AAR-003-F-50µl
市  场 价:¥10540.00
场      地:美国(厂家直采)
产品分类: 生化试剂>生物分子>受体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$527.00
品      牌: Alomone
公      司:Alomone Labs
公司分类:
Alomone/Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody/AAR-003-F/2 x 50 µl
商品介绍
Cat#:AAR-003-F
  • LyophilizedPowder
  • AntigenIncl.
  • ShippedatRoomTemp.
  • Type:Polyclonal
    Source:Rabbit
    Reactivity:h,m
    Mayalsoworkin:r
    Immunogen
    PeptideKDSATNN(S)TEPWDGTTNESC,correspondingtoaminoacidresidues147-166ofhuman A2BAR(Accession P29275).2nd extracellularloop.
    Accession(Uniprot)NumberP29275
    GeneID136
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyRat -14/19aminoacidresiduesidentical;mouse-13/19aminoacidresiduesidentical
    PurityAffinitypurifiedonimmobilizedantigen.
    FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    LabelFluoresceinisothiocyanate(FITC).
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Controlantigenreconstitution100µldoubledistilledwater(DDW).
    Controlantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-003),anddirectflowcytometry(labeledantibody).
    Applications:fc
    Mayalsoworkin:ic,lci
    Directflowcytometry
    DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:
    ___Cellsalone.
    ___Cells+RabbitIgGisotypecontrol-FITC.
    ___Cells+Adenosine A2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.
    DirectflowcytometryofliveintactmouseJ774macrophagecells:
    ___Cellsalone.
    ___Cells+RabbitIgGisotypecontrol-FITC.
    ___Cells+Adenosine A2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.
    References
    1. Peeters,M.C.etal.(2011)FASEBJ.25,632.
    2. Sun,Y.andHuang,P.(2016)Front.Chem.4,37.
    Scientificbackground

    ExtracellularadenosineisaubiquitoussignalingmoleculethatmodulatesawidearrayofBIOLOGicalprocesses.Therearecurrentlyfourknownsub-typesofadenosinereceptors:A1,A2A,A2B andA3.
     
    TheadenosineA2B receptorisatypicalclassA,Gprotein-coupledreceptorwithseventransmembranesegments,anextracellularN-terminusandanintracellularC-terminus.AdenosineA2BRcausesanincreaseinintracellularcAMPlevelsbycouplingmainlytoGs proteins,resultingintheactivationofadenylylcyclase1 andsubsequentactivationofPKAandothercAMPeffectorssuchasEpac.Inaddition,theA2BRcouplestoGq andactivatesthePLCpathwaythatmediatesseveralcrucialfunctionsofA2BR.A2BRalsocouplestotheMAPKandarachidonicacidsignalingpathwaysandregulatesmembraneionchannelsprobablythroughG-proteinβγsubunits.
     
    MultiplemoleculesthatbindtoA2BRhavebeenidentifiedinanefforttounderstandthereceptor’sfunctionandregulation.NFκB1/p105isamemberoftheNFκBfamilyofproteinsthatperformregulatoryfunctionsindiversebiologicalprocessessuchasinflammationandcellsurvivalanddifferentiation,aswellasinvariousdiseases.Interestingly,AlthoughA2BRactivationbyadenosineproducespro-inflammatoryeffectsthereceptoralsobindstospecificsitesonp105,preventsthepoly-ubiquitinationanddegradationofthep105proteinandinhibitsNFκBactivationthusreducinginflammation.
     
    A2BRishighlyexpressedinvarioustypesoftumorcellsortissuesandpromotestumor-cellproliferation.A2BRwasfoundtobeoverexpressedincolorectalcarcinomacellsandtissues,andinhibitionofA2BRblockstheproliferationofcoloncancercells.A2BRhasbeenimplicatedinthepathophysiologyofvasculardiseases,renaldiseaseanddiabetes2.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    Lastupdate:24/03/2019

    Anti-AdenosineA2B Receptor(extracellular)Antibody(#AAR-003)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimaging,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeA2BARfromhuman,rat,andmousesamples.

    Anti-Adenosine A2B Receptor(extracellular)-FITCAntibody(#AAR-003-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibodycanbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryusinglivecells.

    Forresearchpurposesonly,notforhumanuse
    Directflowcytometry
    DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:
    ___Cellsalone.
    ___Cells+RabbitIgGisotypecontrol-FITC.
    ___Cells+Adenosine A2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.
    DirectflowcytometryofliveintactmouseJ774macrophagecells:
    ___Cellsalone.
    ___Cells+RabbitIgGisotypecontrol-FITC.
    ___Cells+Adenosine A2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.
    References
    1. Peeters,M.C.etal.(2011)FASEBJ.25,632.
    2. Sun,Y.andHuang,P.(2016)Front.Chem.4,37.
    品牌介绍
    Alomone成立于1989年,为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂),G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证,以冻干粉形式提供,可确保产品生物活性和保质期。Alomone可提供多种包装规格,灵活方便.
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