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抗体、小分子、蛋白质和肽
℡ 4000-520-616
℡ 4000-520-616
Alomone/Anti-Adenosine A1Receptor Antibody/AAR-006/25 µl
产品编号:AAR-006-25µl
市  场 价:¥4940.00
场      地:美国(厂家直采)
产品分类: 生化试剂>生物分子>受体>
联系QQ:1570468124
电话号码:4000-520-616
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美  元  价:$247.00
品      牌: Alomone
公      司:Alomone Labs
公司分类:
Alomone/Anti-Adenosine A1Receptor Antibody/AAR-006/25 µl
商品介绍
Cat#:AAR-006
  • LyophilizedPowder
  • AntigenIncl.
  • ShippedatRoomTemp.
  • Type:Polyclonal
    Source:Rabbit
    Reactivity:h,m,r
    Immunogen
    Peptide(C)KKVSASSGDPQKYYGKE,correspondingtoaminoacidresidues213-229ofhumanA1AR(Accession P30542).3rd intracellularloop.
    Accession(Uniprot)NumberP30542
    GeneID134
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse,rat,bovine,canisandpig-identical.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25μl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.85mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Controlantigenreconstitution100µldoubledistilledwater(DDW).
    Controlantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1μgpeptideper1μgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,ih,wb
    Mayalsoworkin:ifc,ip
    Westernblot
    Westernblotanalysisofratbrain(lanes1,3)andratkidney(lanes2,4)lysates:
    1,2.Anti-Adenosine AReceptorAntibody(#AAR-006),(1:200).
    3,4.Anti-AdenosineAReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
    Immunohistochemistry
    ExpressionofAdenosine AReceptorinratcortex
    ImmunohistochemicalstainingofratcortexfrozensectionusingAnti-Adenosine AReceptor Antibody(#AAR-006).A.A1AR(green)appearsinneurons(triangles)andinastrocytes(arrows).B.Parvalbuminstaining(red)appearsincorticalinterneurons.C.ConfocalmergeofimagesdemonstratestheexistenceofA1ARinasubsetof corticalinterneuronsandastrocytes.
    ExpressionofAdenosine AReceptorinratmedialseptum
    ImmunohistochemicalstainingofratmedialseptumfrozensectionusingAnti-Adenosine AReceptor Antibody(#AAR-006).A.A1AR(green)appearsinneurons(rightpointingtriangles). B.Parvalbuminstaining(red)appearsinmedialseptalneurons.C.ConfocalmergeofdemonstratesexpressionofA1ARinasubsetofmedial septalneurons.
    Immunocytochemistry
    ExpressionofAdenosine A1 ReceptorinratDRGprimaryculture
    Immunocytochemicalstainingofparaformaldehyde-fixedandpermeABIlizedratdorsalrootganglion(DRG)primaryculture.
    A.Stainingof DRGcellswithAnti-Adenosine AReceptorAntibody(#AAR-006)(1:100),followedbygoatanti-rabbitAlexaFluor-555secondaryantibody.
    B.Nuclearstainingofcellsusingthecell-permeabledyeHoechst33342.
    C.MergedimagesofAandB.
    F.MergedimagesofDandE.
    Magnification:
    A-C:x20
    D-F:x100
    References
    1. Okusa,M.D.(2002)Am.J.Physiol.282,F10.
    2. Nakata,H.(1989)J.Biol.Chem.264,16545.
    3. Linden,J.(1991)FASEBJ.5,2668.
    4. Park,K.S.etal.(2001)J.Pharmacol.Exp.Therap.299,501.
    5. Rice,A.M.etal.(2000)Endocrinology141,1442.
    6. LaNoue,K.F.andMartin,L.F.(1994)FASEBJ.8,72.
    7. Synowitz,M.etal.(2006)CancerRes.66,8550.
    8. Lelievre,V.etal.(1998)Eur.J.Pharmacol.341,289.
    9. Khoo,H.E.etal.(1996)CancerLett.106,17.
    Scientificbackground

    Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.1,2 Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA1 Receptor(A1AR), AdenosineA2A Receptor (A2AAR), AdenosineA2B Receptor (A2BAR),and AdenosineA3 Receptor (A3AR).AllareintegralmembraneproteinsandaremembersoftheGprotein-coupledreceptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,acytoplasmiccarboxylterminus,andathirdintracellularloopthatisimportantforbindingGproteins.1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs.1-3

    A1ARiswidelydistributedandhasbeenwellcharacterized.HighexpressionofA1ARisfoundinthebrain(mainlyinthecortex,cerebellum,andhippocampus),dorsalhornofthespinalcord,adrenalgland,andatria,andtoalowerextentinseveralothertissuesincludingADIposetissue,thecolon,andkidney.2,4

    A1ARmodulatestheactivityofseveralionchannels.ActivationofA1AR(byadenosine,itsmajoragoNIST)inhibitsN-typeCa2+ channelsviaavoltage-dependent,pertussistoxin(PTX)-sensitivepathwayinneuronsoftheratmajorpelvicganglia(MPG).5

    SinceA1ARisthemostprominentadenosinereceptorinadipocytes,ithasbecomeanaturaltargetforresearchonobesity,whichisamajorhealthproblem.6,7 ApossIBLeroleincellproliferationandcarcinogenesishasalsobeensuggestedforA1AR.8-10

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    receivea25µlfreetrialsample!
    Lastupdate:17/02/2019

    AlomoneLabsispleasedtointroduceanewantibodydirectedagainsthumanadenosineAreceptor.Anti-Adenosine A1 ReceptorAntibody(#AAR-006)canbeusedinwesternblotanalysis,immunocytochemicalandimmunohistochemicalapplications.IthasbeendesignedtorecognizeadenosineA1Rfromrat,mouseandhumansamples.

    Forresearchpurposesonly,notforhumanuse
    Westernblot
    Westernblotanalysisofratbrain(lanes1,3)andratkidney(lanes2,4)lysates:
    1,2.Anti-Adenosine AReceptorAntibody(#AAR-006),(1:200).
    3,4.Anti-AdenosineAReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
    品牌介绍
    Alomone成立于1989年,为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂),G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证,以冻干粉形式提供,可确保产品生物活性和保质期。Alomone可提供多种包装规格,灵活方便.
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