Anti-GIPC1Antibody(#APZ-045)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.IthasbeendesignedtorecognizeGIPC1fromhuman,rat,andmousesamples.
产品编号：APZ-045-50µl
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商品介绍
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:h,m,r
Immunogen
Peptide(C)KAPPLVENEEAEPGR,correspondingtoaminoacidresidues10-24ofhumanGIPC1(AccessionO14908).Intracellular,N-terminus.
Accession(Uniprot)NumberO14908
GeneID10755
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat,mouse–14/15aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.8mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefortwoweeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1µgpeptideper1µgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ih,wb
Mayalsoworkin:ic,ip
Westernblot
Westernblotanalysisofratbrainlysate(lanes1and3)andmousebrainmembranes(lanes2and4):
1,2.Anti-GIPC1Antibody(#APZ-045),(1:400).
3,4.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.
3,4.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.
WesternblotanalysisofhumanU87-MGglioblastomacelllinelysate:
1.Anti-GIPC1 Antibody(#APZ-045),(1:400).
2.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.
2.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.
Immunohistochemistry
ExpressionofGIPC1inmouseandrathippocampus
Immunohistochemicalstainingofperfusion-fixedfrozenmouseandratbrainsectionswith Anti-GIPC1Antibody(#APZ-045),(1:200),followedbygoat-anti-rabbit-AlexaFluor-488.A.Inmousebrain,GIPC1staining(green)appearsinneuronsofthepyramidallayer(P)andtheirapicaldendrites(arrows).B.Inratbrainsimilarstainingisobserved.CellnucleiarestainedwithDAPI(blue).
References
- Shang,G.etal.(2017)eLife6,e27322.
- Katoh,M.(2013)Exp.Mol.Med.45,e26.
Scientificbackground
GIPC1belongstoafamilyofevolutionarilyconservedadaptorproteinsresponsIBLeforthetraffickingofnumeroustransmembraneproteinsviaendocytosis.MembersofGIPC(GAIPinteractingprotein,C-terminus)havethreemaindomainsresponsibleforproteininteractions;aPDZdomainwhichisflankedbyGH1andGH2domains.GH1domainisresponsibleforthedimerizationoftheprotein,whileGH2isimportantfortheinteractionofGIPC1withMYO6,aretrogrademotorproteininvolvedinproteintrafficking.ThePDZdomainofGIPC1isresponsiblefortheproteintointeractwithnumerousproteins;namelyβ1-adrenoceptor,D2dopaminereceptor,GLUT1,TrkAreceptorjusttonameafew.ThezebrafishorthologalsointeractswithNeuropilin-21,2.
GIPC1expressionisupregulatedinbreast,ovarianandpancreascancers2.
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:23/10/2018
Forresearchpurposesonly,notforhumanuse
Westernblot
Westernblotanalysisofratbrainlysate(lanes1and3)andmousebrainmembranes(lanes2and4):
1,2.Anti-GIPC1Antibody(#APZ-045),(1:400).
3,4.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.
3,4.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.
WesternblotanalysisofhumanU87-MGglioblastomacelllinelysate:
1.Anti-GIPC1 Antibody(#APZ-045),(1:400).
2.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.
2.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.
品牌介绍
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