Alomone/Anti-α1B-Adrenergic Receptor (extracellular) Antibody/AAR-018/0.2 ml,Alomone,,Alomone,Alomone Labs,Alomone试剂采购 Alomone Labs试剂产品信息,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Alomone/Anti-α1B-Adrenergic Receptor (extracellular) Antibody/AAR-018/0.2 ml

产品编号：AAR-018-0.2ml

市场价：¥9500.00

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>受体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$475.00

品牌： Alomone

公司：Alomone Labs

公司分类：

商品介绍

Cat#:AAR-018

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,r

Mayalsoworkin:m

Immunogen

Peptide(C)KNANFTGPNQTSSNS,correspondingtoaminoacidresidues21-35ofhumanα_1B-adrenoceptor(AccessionP35368).Extracellular,N-terminus.

Anti-α1B-Adrenergic Receptor (extracellular) Antibody -

Accession(Uniprot)NumberP35368

GeneID147

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyMouse-14/15aminoacidresiduesidentical;rat-13/15aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.8mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1μgpeptideper1μgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ic,ifc,ih,lci,wb

Mayalsoworkin:ip

Westernblot

Westernblotanalysisofα_1B-adrenoreceptorinlysatesofratbrain(lanes1and3),ratkidney(lanes2and4)andGH3cellline(lanes5and6):

1,2,5.Anti-α_1B-AdrenergicReceptor(extracellular)Antibody(#AAR-018),(1:200).
3,4,6.Anti-α_1B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

Expressionofα_1B-Adrenoreceptorinratcerebellum

ImmunohistochemicalstainingoffrozensectionofratcerebellumusingAnti-α_1B-AdrenergicReceptor(extracellular)Antibody (#AAR-018),(1:100).α_1B-Adrenoceptor(red)isdetectedinPurkinjecells.Hoechst33342wasusedasthecounterstain.

Indirectflowcytometry

IndirectflowcytometryanalysisofGH3livingcells:

^___Unstainedcells.
^___Cells+Anti-α_1B-AdrenergicReceptor(extracellular)Antibody(#AAR-018),(10µg/5x10⁵cells).

Thecontrolantigenisnotsuitableforthisapplication.

Livecellimaging/Immunocytochemistry

Expressionofα_1B-AdrenoceptorinGH3cells

Cellsurfacedetectionof α_1B-AdrenoceptorinlivingGH3cellswithAnti-α_1B-AdrenergicReceptor (extracellular)Antibody (#AAR-018),followedbygoatanti-rabbit-AlexaFluor-488secondaryantibody(green).NuclearstainingofcellsusingtheDNAdyeHoechst33342(blue).

References

IUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Perez,D.M.etal.(1993)Mol.Pharmacol.44,784.
Minneman,K.P.(1988)Pharmacol.Rev.40,87.
Schwinn,D.A.etal.(2004)MayoClin.Proc.79,1423.

Scientificbackground

Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.

Adrenergicreceptorsaremembersofthe G-proteincoupledreceptor (GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.

Adrenoceptorsaredividedintothreetypes:α₁,α₂ andβadrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α_1A,α_1B,α_1D,α_2A,α_2B,α_2C,β₁,β₂,β₃.^1,2Theyareexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.^1,2

Allα₁-adrenoceptors(α₁-ARs)activatephospholipasesCandA2.³ Inadditiontomobilizingintracellularcalcium,theα₁-ARshavealsobeenshowntoactivatecalciuminfluxviavoltage-dependentand-independentcalciumchannels.⁴

α_1B-Adrenergicreceptorpopulationsaregreatestinthespleen,kidney,cerebellum,andfetalbrain.⁵

α_1B-Adrenoceptorcausescontractionofsmoothmusclecellsandtherebycontrolsvasculartone,bloodpressure,andacceleratesthedevelopmentofatherosclerosis.⁵

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea50µlfreetrialsample!

Lastupdate:19/03/2019

Anti-α_1B^–AdrenergicReceptor(extracellular)Antibody(#AAR-018)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanα_1B-adrenoceptor.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimagingandindirectflowcytometryapplications.Ithasbeendesignedtorecognizeα_1B-adrenoceptorfromhuman,ratandmousesamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

Westernblotanalysisofα_1B-adrenoreceptorinlysatesofratbrain(lanes1and3),ratkidney(lanes2and4)andGH3cellline(lanes5and6):

1,2,5.Anti-α_1B-AdrenergicReceptor(extracellular)Antibody(#AAR-018),(1:200).
3,4,6.Anti-α_1B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone成立于1989年，为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂)，G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便.

公司介绍

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产品分类

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Adenosine Receptors Adhesion Class GPCRs Acid-sensing G-protein Coupled Receptors Adrenergic Receptors

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