Alomone/Anti-α1D-Adrenergic Receptor (extracellular) Antibody/AAR-019/50 µl,Alomone,,Alomone,Alomone Labs,品牌试剂实验Alomone代理 Alomone G蛋白偶联受体GPCR,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Alomone/Anti-α1D-Adrenergic Receptor (extracellular) Antibody/AAR-019/50 µl

产品编号：AAR-019-50µl

市场价：¥7340.00

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>受体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$367.00

品牌： Alomone

公司：Alomone Labs

公司分类：

商品介绍

Cat#:AAR-019

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,m,r

Immunogen

Peptide(C)EPVPPDERF*SGITEE,correspondingtoaminoacidresidues231-245ofratα_1D-adrenoceptorwithreplacementofcysteine240(C₂₄₀)withserine(*S)(AccessionP23944).3^rdextracellularloop.

Anti-α1D-Adrenergic Receptor (extracellular) Antibody -

Accession(Uniprot)NumberP23944

GeneID29413

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyRat,human,mouse-14/15aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedanitbodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.

Antibodyconcentrationafterreconstitution0.8mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1μgpeptideper1μgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis.

Applications:ic,ifc,ih,lci,wb

Mayalsoworkin:ip

Westernblot

Westernblotanalysisofratbrainstem(lanes1and3),mousebrain(lanes2and4),SH-SY5Y(lanes5and7)andJurkat(lanes6and8)lysates:

1,2,5,6.Anti-α_1D-AdrenergicReceptor(extracellular) Antibody(#AAR-019),(1:200).
3,4,7,8.Anti-α_1D-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

Immunohistochemistry

Expressionofα_1D-Adrenoceptorinratheart

ImmunohistochemicalstainingofratheartparaffinembeddedsectionsusingAnti-α_1D-AdrenergicReceptor(extracellular)Antibody(#AAR-019),(1:100).α_1D-Adrenoceptorisexpressedincardiomyocytesofthemyocardiumandinthesmoothmuscleofthebloodvessels(arrows).Hematoxilinisusedasthecounterstain.

Expressionofα_1D-Adrenoceptorinratneocortex

Immunohistochemicalstainingofα_1D-adrenoceptorinratneocortexusingAnti-α_1D-AdrenergicReceptor(extracellular)Antibody(#AAR-019). A.Mostintensestainingofα_1D-adrenoceptor(green)appearsinapicaldendrites(thinhorizontalarrows)butalsointhesoma(thickhorizontalarrow).Fewcorticalinterneuronsexpressα_1D-adrenoceptor.B.Thesamesectionwasalsostainedforparvalbuminandonecell(markedwithaverticalarrow)alsoexpressesα_1D-adrenoceptor.

Indirectflowcytometry

IndirectflowcytometryanalysisofintactliveJurkatcells:

^___Unstainedcells.
^___Cells+Anti-α_1D-AdrenergicReceptor(extracellular) Antibody(#AAR-019),(5-10µgantibody/0.5x10⁶cells).

Thecontrolantigenisnotsuitableforthisapplication.

Livecellimaging/Immunocytochemistry

Expressionofα_1D-Adrenoceptor inratPC12cells

Cellsurfacedetectionof α_1D-AdrenoceptorinintactlivingratpheochromocytomaPC12cells.A.ExtracellularstainingofcellsusingAnti-α_1D-AdrenergicReceptor (extracellular) Antibody(#AAR-019)(1:100) followedbygoatanti-rabbit-AlexaFluor-594secondaryantibody.B.MergeofAandliveviewofthecells.

References

IUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Chen,Z.J.andMinneman,K.P.(2005)ActaPharmacol.Sin.26,1281.
Tanoue,A.etal.(2002)J.Clin.Invest.109,765.
Chen,Q.etal.(2005)J.Urol.174,370.

Scientificbackground

Adrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.

AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.

Adrenoceptorsaredividedintothreetypes:α₁,α₂ andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes: α_1A, α_1B,α_1D, α_2A, α_2B, α_2C,β₁, β₂, β₃^1,2.Theadrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem^1,2.

α₁-Adrenergicreceptorsplayanimportantroleinthephysiologicalresponsetoepinephrineandnorepinephrine,particularlyinthecardiovascularsystem.Allthreeclonedα₁ receptors(α_1A,α_1B,andα_1D)coupletoG_q/11.Whiletheircellulardistributionisclear,theirfunctionalroleislesssoduetoalackofspecificagoNISTs/antagonists³.

Asthesereceptorslackspecificagonists/antagonists,knock-out(KO)studiesinmicehaveshedsignificantlightontheircellularrole.Individualα₁-adrenoceptorKOmicedonotdisplayphysicalabnormalitiesingeneral.SimilarKOstudiesindicatethatallα₁ subtypesseemtobeinvolvedintheregulationofbloodpressure³.Also,studiesindicatethatα_1D-adrenoceptorseemstoplayanimportantroleinaorticcontraction⁴,renalfunction,nociception,basallocomotoractivity³ aswellasbladdercontraction⁵.

LyophilizedPowderControlAntigenIncluded

Clickheretoreceivea50µlfreetrialsample!

Lastupdate:19/03/2019

AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanextracellularepitopeoftheratα_1D-adrenergicreceptor.Anti-α_1D-AdrenergicReceptor(extracellular)Antibody(#AAR-019)canbeusedinwesternblot,indirectflowcytometryandimmunohistochemicalapplications.Ithasbeendesignedtorecognizeα_1D-adrenoceptorfrommouse,ratandhumansamples.

Forresearchpurposesonly,notforhumanuse

Westernblot

Westernblotanalysisofratbrainstem(lanes1and3),mousebrain(lanes2and4),SH-SY5Y(lanes5and7)andJurkat(lanes6and8)lysates:

1,2,5,6.Anti-α_1D-AdrenergicReceptor(extracellular) Antibody(#AAR-019),(1:200).
3,4,7,8.Anti-α_1D-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

品牌介绍

Alomone成立于1989年，为全球科学家提供最全面的离子通道研究工具。Alomone是离子通道 (多种离子通道抗体及调节剂)，G蛋白偶联受体GPCR (抗体、激动剂、拮抗剂) 与神经信号传导 (抗体、神经营养因子) 研究工具的领先供应商。产品主要为抗体、小分子、蛋白质和肽。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便.

公司介绍

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产品分类

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Adenosine Receptors Adhesion Class GPCRs Acid-sensing G-protein Coupled Receptors Adrenergic Receptors

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